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New calcium fluorescent probes
FLUORESCENT RED EMITTING FUNCTIONALIZABLE CALCIUM INDICATORS
Inventors: Jean-Maurice Mallet, Mayeul Collot
Laboratory: Laboratoire des Biomolécules de l'École Normale Supérieure, UMR8247.
Technical domain: Life technology
Intellectual property: PCT/EP/2014/075766
Calcium Rubies-Nano are new calcium fluorescent probes and have been developed to meet the different needs of marking in biology, and simultaneous two-color markings. These innovative probes for imaging are able to emit in the red (> 600 nm). They have the advantage of being very high affinity (Kd = 258 nM) and include a functionalisable arm. These probes retain their physicochemical properties after linking with their target.
Using two-photon microscopy and simultaneous patch-clamp recording, results give signals comparable to commonly used green emitting [Ca2+] probes.
High-speed random access microscopy is used with Purkinje cells filled in cerebellar slices with CaRu-Nano-Dextran-6000 conjugate and Alexa Fluor-488. Reports [Ca2+] transients with kinetics are comparable to commonly used indicators.
Calcium is involved in many biological processes. The applications of the new family of probes are numerous, in particular, cellular imaging for neuroscience, virology, cardiology, oncology...